Effects of Chronic Nicotine Administration on Salivary Gland in Mice: Immunohistochemical Study
정정우, 최재갑, 변진석,
소속 상세정보
정정우 ( Jung Jung-Woo ) - Kyungpook National University School of Dentistry Department of Oral Medicine
최재갑 ( Choi Jae-Kap ) - Kyungpook National University School of Dentistry Department of Oral Medicine
변진석 ( Byun Jin-Seok ) - Kyungpook National University School of Dentistry Department of Oral Medicine
KMID : 0355220140390030083
Abstract
Purpose: Chronic nicotine administration induce various effects in whole organs of the body; however, little is known about salivary gland. In the present study, we pursued the links between systemic nicotine and the histomorphological changes of the salivary gland in mice.
Methods: Twenty-five C57BL6 mice were allocated into two groups. The control group (n=9) received distilled water only for 8 weeks by gavage. The experimental nicotine group (n=16) was administered nicotine 5 μg/g with distilled water. Animals were sacrificed at 8 weeks; then, submandibular glands were excised and processed for histologic evaluation. Volumetric changes in acinar cells were evaluated by H&E staining. The expression of calponin-positive myoepithelial cells and Ki-67-positive proliferating acinar cells were evaluated by immunohistochemistry.
Results: The nicotine group showed significantly decreased number of calponin-positive myoepithelial cell process compared with the control group. There were no significant differences in average volume of acinar cell and the number of Ki-67-positive acinar cells between both groups.
Conclusions: These findings suggested that chronic nicotine administration may cause decreased function of myoepithelial cells in submandibular glands of mice, and these can partly explain xerostomic conditions in chronic smokers.
키워드
Acinar cells; Calponin; Myoepithelial cells; Nicotine; Submandibular gland; Xerostomia
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