Expression of Amino Acid Transporter LAT1 During Ameloblast Differentiation
김상봉, 김도경, 김춘성, 국중기, 박주철, 김흥중,
소속 상세정보
김상봉 ( Kim Sang-Bong ) - 조선대학교 치과대학 구강생물학연구소
김도경 ( Kim Do-Kyung ) - 조선대학교 치과대학 구강생물학연구소
김춘성 ( Kim Chun-Sung ) - 조선대학교 치과대학 구강생리학교실
국중기 ( Kook Joong-Ki ) - 조선대학교 치과대학 구강생물학연구소
박주철 ( Park Ju-Cheol ) - 서울대학교 치과대학 발생생물,구강조직학교실
김흥중 ( Kim Heung-Joong ) - 조선대학교 치과대학 구강생물학연구소
KMID : 0848120090340030143
Abstract
Amino acid transporters play important roles in supplying nutrients to cells. In our current study, we investigated the expression of LAT1 and measured the amino acid uptake in ameloblast cultures to further elucidate the roles of this transporter during the differentiation of these cells. RT-PCR, observations of cell morphology, Alizaline red-S staining, and uptake analyses were performed following the experimental induction of differentiation in the cultures. LAT1 mRNA was detectable and found to gradually increase over time whereas LAT2 mRNA was not evident in the ameloblast cultures. Transcripts of 4F2hc, a cofactor of LAT1 and LAT2, were also found to be expressed in ameloblast cultures and increase with time. Amelogenin mRNA was expressed in the early stage ameloblast cultures. L-leucine uptake was observed to increase over 14 days of growth in culture. Our data suggest that LAT1 has a key role in the differentiation of ameloblasts and in providing these cells with neutral amino acids, including several essential amino acids.
키워드
amino acid transporter;LAT1;ameloblast;differentiation
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