Apoptotic Activity of Curcumin and EF-24 in HTB-41 Human Salivary Gland Epidermoid Carcinoma Cells
김지원, 이슬아, 고대산, 박병선, 김수관, 유선경, 오지수, 김춘성, 김정선, 박종태, 김도경,
소속 상세정보
김지원 ( Kim Ji-Won ) - Chosun University School of Dentistry Oral Biology Research Institute
이슬아 ( Lee Seul-Ah ) - Chosun University School of Dentistry Oral Biology Research Institute
고대산 ( Go Dae-San ) - Chosun University School of Dentistry Oral Biology Research Institute
박병선 ( Park Byung-Sun ) - Chosun University School of Dentistry Oral Biology Research Institute
김수관 ( Kim Su-Gwan ) - Chosun University School of Dentistry Oral Biology Research Institute
유선경 ( Yu Sun-Kyoung ) - Chosun University School of Dentistry Oral Biology Research Institute
오지수 ( Oh Ji-Su ) - Chosun University School of Dentistry Oral Biology Research Institute
김춘성 ( Kim Chun-Sung ) - Chosun University School of Dentistry Oral Biology Research Institute
김정선 ( Kim Jeong-Sun ) - Gwangju Health University Department of Dental Hygiene
박종태 ( Park Jong-Tae ) - Dankook University College of Dentistry Department of Oral Anatomy
김도경 ( Kim Do-Kyung ) - Chosun University School of Dentistry Oral Biology Research Institute
KMID : 0848120150400020063
Abstract
Curcumin (diferuloylmethane), a constituent of turmeric powder derived from the rhizome of Curcuma longa, has been shown to inhibit the growth of various types of cancer cells by regulating cell proliferation and apoptosis. However, a need exists to design more effective analogs because of curcumin’s poor intestinal absorption. EF-24 (diphenyl difluoroketone), the monoketone analog of curcumin, has shown good efficacy in anticancer screens. However, the effects of curcumin and EF-24 on salivary gland epidermoid carcinoma cells are not clearly established. The main goal of this study was to investigate the effects of curcumin and EF-24 on cell growth and induction of apoptosis in human salivary gland epidermoid carcinoma cells. Our studies showed that curcumin and EF-24 inhibited the growth of HTB-41 cells in a dose- and time-dependent manner, and the potency of EF-24 was >34-fold that of curcumin. Treatment with curcumin or EF-24 resulted in nuclear condensation and fragmentation in HTB-41 cells, whereas the control HTB-41 cell nuclei retained their normal regular and oval shape. Curcumin and EF-24 promoted proteolytic cleavages of procaspase-3/-7/-9, resulting in an increase in the amount of cleaved caspase-3/-7/-9 in the HTB-41 cells. Caspase-3 and -7 activities were detected in viable HTB-41 cells treated with curcumin or EF-24. These results suggest that the curcumin and EF-24 inhibit cell proliferation and induce apoptosis in HTB-41 human salivary gland epidermoid carcinoma cells, and that they may have potential properties as an anti-cancer drug therapy.
키워드
EF-24 ; curcumin ; cell death ; apoptosis ; salivary gland epidermoid carcinoma cells
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