ODAM과 BMPRIB가 법랑질의 석회화에 미치는 영향
Effect of ODAM and BMPRIB on Enamel Mineralization
박종태, 조광희, 배현숙, 조영식, 김흥중,
소속 상세정보
박종태 ( Park Jong-Tae ) - 연세대학교 치과대학 구강생물학교실
조광희 ( Cho Kwang-Hee ) - 조선대학교 치과대학 구강해부학교실
배현숙 ( Bae Hyun-Sook ) - 남서울대학교 치위생학과
조영식 ( Cho Young-Sik ) - 남서울대학교 치위생학과
김흥중 ( Kim Heung-Joong ) - 조선대학교 치과대학 구강해부학교실
KMID : 1023420110110010055
Abstract
The purpose of this study was to investigate the biological function of ODAM and its signal transduction pathway in the steps of ameloblast differentiation and enamel mineralization. An ODAM recombinant protein was produced and stable ODAM transgenic cell lines were also established using ameloblast-lineage cells (ALCs). To verify the ODAM signal transduction pathway, BAMBI recombinant protein, an inhibitor of BMP2 and BMP receptor 1B (BMPR-1B), was treated and BMPR-1B siRNA was used to silence expression of BMPR-1B. Mineralization was augmented by the ALCs treated with the ODAM recombinant protein and the sense ODAM overexpressing cells. The ALP activity was also increased markedly in the sense ODAM overexpressing cells and the ALCs treated with ODAM recombinant protein. The inactivation of ODAM in the ALCs down-regulated the expression of BMPR-1B, whereas its expression was up-regulated markedly when ODAM was overexpressed. These results provide deeper insights into the process of ameloblast maturation and in enamel mineralization. It also suggested that ODAM augmented enamel mineralization.
키워드
Ameloblast; Enamel; Mineralization; Odontogenic ameloblast-associated protein (ODAM); Signal transduction system
원문 및 링크아웃 정보
등재저널 정보