Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry
김지아, 송영상, 민경산, 김선헌, 고정태, 이빈나, 장훈상, 황인남, 오원만, 황윤찬,
소속 상세정보
김지아 ( Kim Ji-A ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
송영상 ( Song Young-Sang ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
민경산 ( Min Kyung-San ) - Chonbuk National University School of Dentistry Department of Conservative Dentistry
김선헌 ( Kim Sun-Hun ) - Chonnam National University Research Center for Biomineralization Disorders
고정태 ( Koh Jeong-Tae ) - Chonnam National University Research Center for Biomineralization Disorders
이빈나 ( Lee Bin-Na ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
장훈상 ( Chang Hoon-Sang ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
황인남 ( Hwang In-Nam ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
오원만 ( Oh Won-Mann ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
황윤찬 ( Hwang Yun-Chan ) - Chonnam National University School of Dentistry Department of Conservative Dentistry
KMID : 1034420160410010029
Abstract
Objectives: The purpose of this study was to assess the ability of two new calcium silicate-based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA).
Materials and Methods: Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP).
Results: On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP.
Conclusions: Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.
키워드
BioAggregate; Biodentine; Dentin sialoprotein; Micro-CT; MTA
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