A NELL-1 Binding Protein: Vimentin
채화성, 김영호,
소속 상세정보
채화성 ( Chae Hwa-Sung ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Orthodontics
김영호 ( Kim Young-Ho ) - Sungkyunkwan University School of Medicine Samsung Medical Center Department of Orthodontics
Abstract
Purpose: Craniosynostosis (CS), one of the most common congenital craniofacial deformities, is the premature closure of cranial sutures. NELL-1 is a novel molecule overexpressed during premature cranial suture closure in human CS. From a functional perspective, NELL-1 has been reported to accelerate chondrocyte maturation and modulate calvarial osteoblast differentiation and apoptosis pathways. The mechanism through which NELL-1 induces these phenomena, however, remains unclear. The purpose of this study is to identify the NELL-1 binding protein(s) through which the biologic mechanism of NELL-1 can be further investigated.
Materials and Methods: Far-Western and Immunoprecipitation (IP) assays were performed, independently and in sequence, followed by mass spectrometry to identify the NELL-1 binding proteins. Reverse IP was used to verify and confirm candidate binding protein.
Results: The only confirmative protein from current experimentation was vimentin. Vimentin is the major structural component of the intermediate filaments.
Conclusion: The present study identified and confirmed vimentin as a NELL-1 binding protein, which opened up a new window to mechanistically facilitate studies on this CS-associated molecule.
키워드
NELL-1 protein;Vimentin;Far-Western blotting;Mass spectrometry
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