Tetrodotoxin (TTX)의 Streptococcus mutans와 Staphylococcus aureus에 대한 생육 감수성
Growth Susceptibility of Tetrodotoxin (TTX), Pufferfish, Against Streptococcus mutans and Staphylococcus aureus
김현대, 김세연,
소속 상세정보
김현대 ( Kim Hyun-Dae ) - 동부산대학교 치위생과
김세연 ( Kim Se-Yeon ) - 부산대학교 치의학전문대학원 구강미생물학교실
KMID : 1143520160040020076
Abstract
The objective of this study is to search the possibility of using the liver of pufferfish, a marine creature as natural antimicrobial by experimenting flesh sensitivity of tetrodotoxin extracted from the liver of pufferfish, a marine creature to Streptococcus mutans and Staphylococcus aureus . The tetrodonic acid (TDA), namely, inducer of tetrodotoxin was confirmed with High Performance Liquid Chromatography (HPLC) to detect and extract “tetrodotoxins fraction” of pufferfish. In order to measure the effect of tetrodotoxin on the growth of Streptococcus mutans and Staphylococcus aureus , it is added to each culture at each concentration of 0-10 nM. After they are measured with spectrophotometer, it is found that experimental group shows the power to inhibit the growth of Streptococcus mutans in 30 hours after cultivation in comparison with control group. Then, the growth curve of Staphylococcus aureus is in the similar type to that of S. mutans . It is found that the growth of S. aureus is inhibited in 24 hours after cultivation in the experimental group where 0.01?0.07 nM tetrodotoxin is contained. Further, the experimental group where 0.15-10 nM tetrodotoxin is contained mostly shows the power to inhibit strain in 4 hours after cultivation, more apparent than the growth inhibiting power of S. mutans . When measuring pH after cultivating experimental group in Streptococcus mutans culture for 24 hours by adding tetrodotoxin of each concentration, we found pH 7.4-7.7 by 4 hours before cultivation. The pH did not vary apparently in comparison to control group. When the contents of tetrodotoxin in each concentration increase in the S. aureus culture, the change in pH is not large in comparison to cultivation time. However, it rapidly decreased in experimental group in just 6 hours after cultivation. Thus, it is judged that it works to hinder the strain growth. As a result, tetrodotoxin is judged to inhibit the growth of Streptococcus mutans and Staphylococcus aureus.
키워드
Growth susceptibility; Tetrodotoxin; Streptococcus mutans; Streptococcus aureus
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