치근단 병소가 있는 한자에서 Porphyromonas endodontalis 항원에 대한 혈청특이 항체의 면역 반응 연구
Immune reaction of specific serum antibodies to porphyromonas endodontalis antigen in patients with periapical lesion
김재희, 高福子,
소속 상세정보
김재희 ( ) - 서울대학교
高福子 ( ) - 서울대학교
KMID : 0362319940190020485
Abstract
Porphyromonas endodontalis is a black-pigmented anaerobic Gram-negative rod which is associated with endodontal infections and this microorganism possesses a potential for pathogenicity. The purpose of this study was to compare the membrane
components
of Porphyromonas endodontalis and Porphyromonas gingivalis and to study the immune reaction patterns of Porphyromonas endodontalis with patients with periapical lesion. Porphyromonas endodontalis (ATCC 35406), Porphyromonas gingivals
serotypea(381),
serotype b(W50), serotype c(A7A1-28) were cultured in anaerobic condition. Rabbit antisera were prepared by intravenous injection of formalized whole cells and human sera were obtained from patients and dental students. Indirect
immunofluorescence
method was used to study on the cross reaction between Porphyromonas endodontalis and Porphyromonas gingivalis serotype a, b, c antigen. Total membrane protein profiles of Porphyromonas endodontalis antigen were studied by sodium dodecyl sulfate
polyacrylamide gel electrophoresis and the reactivity of antigenic components of Porphyromonas endodontalis against sera of patients and rabbit anti-Porphyromonas endodontalis antisera were assessed by Immunoblotting method.
@ES The following results were obtained :
@EN 1. Antigens of Porphyromonas endodontalis has multiple antigenic components, and both patients with periapical lesion and normal healthy individual showed immune response to this.
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