Capsaicin이 연수후각의 흥분성 아미노산 전달물질에 미치는 영향
Effect of capsaicin on the excitatory amino acids neurotranmitters in medullary dorsal horn
권수경, 高福子, 엄보용,
소속 상세정보
권수경 ( ) - 서울대학교
高福子 ( ) - 서울대학교
엄보용 ( ) - 서울대학교
KMID : 0362319940190020621
Abstract
This experiment was performed to study the effect of capsaicin on the excitatory amino acids (EAAs) neurotransmitter in medullary dorsal horn and to clarify the relationship between substance P and excitatory amino acids. Horizontal slice of rat
medullary dorsal horn was prepared and perfused with modified Krebs-Ringer solution in brain slice chamber. Release of EAAs was induced by veratrine and capsaicin were added to perfusion solution to observe the changes in EAA release. Capsaicin
and
ruthenium red, capsaicin antagonist, were also systemically injected with 50mg/kg in first day and 100mg/kg in second day for 2 days. Medulla oblongata containing the medullary dorsal horn was isolated, homogenized and centrifused. Spernatant was
freeze-dried and EAA was determined by HPLC. Release of glutamate and aspartate was significantly increased by veratrine or capsaicin, but veratrine evoked release of EAAs was blocked by capsaicin in vitro, and injected ruthenium red did not have
effect
on the contents of EAAs in vivo. Systemically injected capsaicin evoked the slight decrease in content of glutamate and aspartate in medullary dorsal horn and this effect of capsaicin was unaffected by ruthenium red.
키워드
원문 및 링크아웃 정보
등재저널 정보