연쇄구균의 세포벽 단백질 추출물이 림프구 활성의 억제에 미치는 영향
The inhibitory effect of streptococcal cell wall extracts on stimulation of lymphocytes
상현숙, 정희일, 오세홍, 양영채,
소속 상세정보
상현숙 ( ) - 원광대학교
정희일 ( ) - 원광대학교
오세홍 ( ) - 원광대학교
양영채 ( ) - 원광대학교
KMID : 0362319950200010275
Abstract
The inhibitory effect of cell wall extracts of streptococci, have been investigated to know host-parasite relationship or pathogenesis of abscess formation.
Streptococci isolated from the infected root canals were sonicated to get cell wall extracts which have been known as oe of the factors of pyogenesis. Lymphocytes separated by density gradient were stimulted with phytohemagglutinin and exposed to
cell
wall extracts of Streptococcus sanguis, S. mitis, S. uberis, S. mutans (ATCC 10449), and S. faecalis (ATCC 19433). [3H]-thymidine uptake of lymphocytes was analyzed with scintillation counter and lactate dehyrogenase (LD) activity was measured
with
autochemistry analyzer.
S. faeealis had the strongest inhibitory effect. Beginning at 100 ㎍/ml concentration of sonic extracts. S. sanguis and S. mitis had inhibitory effect at 300 ㎍/ml, while S. uberis and S. mutans showed no inhibitory effect on DNA syntheis even at
300㎍/ml.
Each streptococci showed different inhibitory effect on the DNA synthesis of lymphocytes, which finding indicated wide spectrum of susceptibility of lymphocytes according to streptococcus spp. There were no significant difference of LD activities
between control and each streptococcal extracts. Streptococcal sonic extracts did not affect the morphological findings or number of colonies activated lymphocytes. These finding suggested the inhibitory effect of sonic extract of streptococci to
lymphocytes could be detected by DNA synthesis inhibition, not by cellular membrane damage.
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