세균액 및 세균단백질 추출물이 배양 세포에 미치는 영향
EFFECTS OF HEAT-KILLED AND SONIC EXTRACTS OF MICROORGANISM ON CULTURED CELLS
소속 상세정보
유영대
임미경
KMID : 0362320000250040606
Abstract
Dental pulp infection is most commonly caused by extensive dental caries, and some bacterial species invade root canals; bacterial components and products are thought to be associated with the pathogenesis of periapical periodontitis. A principle driving force behind pulpal disease response appears to lie in the host immune system´s to bacteria and their products. We examined the production of interleukin 1β (IL-1β) and tumor necrosis factor α(TNF-α) from human peripheral mononuclear cells, lymphocytes and monocytes stimulated by heat-killed Acitnobacillus actinomycetemcomitans (ATCC 29523), Porphyromonas gingivalis (ATCC 33277) and Prevotella intermedia (ATCC 25611), and also by their sonicated bacterial extracts(SBE), respectively. The effects of three strains of heat-killed bacteria and their SBEs on the morphology of cultured blood cell lines HL-60 (KCLB 10240) and J774A.1 (KCLB 40067) were observed under the inverted microscope. Ultrastructural changes of J774A.1 exposed to heat- killed P. intermedia and its SBE were investigated using transmission electron microscopy. Production of IL-1β was reduced in human peripheral mononuclear cells after stimulation by sonic bacterial extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. Heat- killed and Sonic extract of P. gingivalis inhibited the production of TNF-α in peripheral mononuclear cells. Production of TNF-α was inhibited in peripheral monocytes after stimulation by sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia. HL-60 and J 774A.1 cells showed granular degeneration after treatment with heat- killed and sonic extracts of A. actinomycetemcomitans, P. gingivalis, and P. intermedia Chromatin margination and shrinkage were observed in 774A.1 treated with heat-killed P. intermedia. Cell wall structure and organelles were destroyed and vacuoles were formed in cytoplasm in J774A.1 treated with P. intermedia sonic extract.
These results suggest that A. actinomycetemcomitans, P. gingivalis and P. intermedia may have an important role in the formation and progression of pulpal diseases via both modulation of production of IL-1β and TNF-α from blood mononuclear cells and cytopathic effects.
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