Microbial profile of asymptomatic and symptomatic teeth with primary endodontic infections by pyrosequencing
임상민, 이태권, 김은정, 박준홍, 이윤, 배광식, 금기연,
소속 상세정보
임상민 ( Lim Sang-Min ) - Seoul National University School of Dentistry Department of Conservative Dentistry
이태권 ( Lee Tae-Kwon ) - Yonsei University Department of Civil and Environmental Engineering
김은정 ( Kim Eun-Jeong ) - Seoul National University School of Dentistry Department of Conservative Dentistry
박준홍 ( Park Joon-Hong ) - Yonsei University Department of Civil and Environmental Engineering
이윤 ( Lee Yoon ) - Yonsei University Wonju College of Medicine Department of Dentistry
배광식 ( Bae Kwang-Shik ) - Seoul National University School of Dentistry Department of Conservative Dentistry
금기연 ( Kum Kee-Yeon ) - Seoul National University School of Dentistry Department of Conservative Dentistry
KMID : 0362320110360060498
Abstract
Objectives:The purpose of this in vivo study was to investigate the microbial diversity in symptomatic and asymptomatic canals with primary endodontic infections by using GS FLX Titanium pyrosequencing.
Materials and Methods:Sequencing was performed on 6 teeth (symptomatic, n = 3; asymptomatic, n = 3) with primary endodontic infections. Amplicons from hypervariable region of the small-subunit ribosomal RNA gene were generated by polymerized chain reaction (PCR), and sequenced by means of the GS FLX Titanium pyrosequencing
Results:On average, 10,639 and 45,455 16S rRNA sequences for asymptomatic and symptomatic teeth were obtained, respectively. Based on Ribosomal Database Project Classifier analysis, pyrosequencing identified the 141 bacterial genera in 13 phyla. The vast majority of sequences belonged to one of the seven phyla: Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, Proteobacteria, Spirochetes, and Synergistetes. In genus level, Pyramidobacter, Streptococcus, and Leptotrichia constituted about 50% of microbial profile in asymptomatic teeth, whereas Neisseria, Propionibacterium, and Tessaracoccus were frequently found in symptomatic teeth (69%). Grouping the sequences in operational taxonomic units (3%) yielded 450 and 1,997 species level phylotypes in asymptomatic and symptomatic teeth, respectively. The total bacteria counts were significantly higher in symptomatic teeth than that of asymptomatic teeth (p < 0.05).
Conclusions:GS FLX Titanium pyrosequencing could reveal a previously unidentified high bacterial diversity in primary endodontic infections.
키워드
Asymptomatic; Primary apical periodontitis; Pyrosequencing; Symptomatic; Taxonomy; 16S rRNA gene
원문 및 링크아웃 정보
등재저널 정보