Inhibitory effect on Streptococcus mutans and mechanical properties of the chitosan containing composite resin
김지선, 신동훈,
소속 상세정보
김지선 ( Kim Ji-Sun ) - Dankook University College of Dentistry and Institute of Dental Science Department of Conservative Dentistry
신동훈 ( Shin Dong-Hoon ) - Dankook University College of Dentistry and Institute of Dental Science Department of Conservative Dentistry
KMID : 0362320130380010036
Abstract
Objectives: This study evaluated the antibacterial effect and mechanical properties of composite resins (LCR, MCR, HCR) incorporating chitosan with three different molecular weights (L, Low; M, Medium; H, High).
Materials and Methods: Streptococcus (S). mutans 100 mL and each chitosan powder were inoculated in sterilized 10 mL Brain-Heart Infusion (BHI) solution, and was centrifuged for 12 hr. Absorbance of the supernatent was measured at OD660 to estimate the antibacterial activities of chitosan. After S. mutans was inoculated in the disc shaped chitosan-containing composite resins, the disc was cleansed with BHI and diluted with serial dilution method. S. mutans was spread on Mitis-salivarius bacitracin agar. After then, colony forming unit (CFU) was measured to verify the inhibitory effect on S. mutans biofilm. To ascertain the effect on the mechanical properties of composite resin, 3-point bending and Vickers hardness tests were done after 1 and 3 wk water storage, respectively. Using 2-way analysis of variance (ANOVA) and Scheffe test, statistical analysis was done with 95% significance level.
Results: All chitosan powder showed inhibition effect against S. mutans. CFU number in chitosan-containing composite resins was smaller than that of control resin without chitosan. The chitosan containing composite resins did not show any significant difference in flexural strength and Vickers hardness in comparison with the control resin. However, the composite resin, MCR showed a slightly decreased flexural strength and the maximum load than those of control and the other composite resins HCR and LCR.
Conclusions: LCR and HCR would be recommended as a feasible antibacterial restorative due to its antibacterial nature and mechanical properties.
키워드
Antibacterial effect; Chitosan; Composite resin; Flexural strength; Vickers hardness
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