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Titanium disc에 도포한 부착단백질이 사람 치은 조섬유세포의 부착 및 증식에 미치는 영향
THE EFFECT OF ATTACHMENT PROTEINS COATED ON TITANIUM DISCS ON THE ADHESIVENESS AND PROLIFERATION OF HUMAN GINGIVAL FIBRO BLAST

대한치과이식학회지 1993년 13권 1호 p.82 ~ 109

최정원, 강윤선, 이근우,

소속 상세정보

최정원 ( Choi Jeung-Won ) - Yonsei University College of Dentistry Department of Prosthodontics
강윤선 ( Kang Yun-Seon ) - Yonsei University College of Dentistry Department of Oral Biology
이근우 ( Lee Keun-Woo ) - Yonsei University College of Dentistry Department of Prosthodontics

KMID : 0362819930130010082

Abstract

Titanium disc에 도포한 부착단백질이 사람 치은

Endosseous dental implants are interfaced with bone, connective tissue, and epithelium when implanted into the jaw bone. The soft tissue interface including connective tissue and epithelium is one of the most critical factors in the determination of implant maintenance and prognosis. The connecdtive tissue interface not only forms the attachment apparatus, but also contributes to the formation of healthy peri-implant tissue by prevention of the epithelial downgrowth. There are two ways to promote connective tissue fibroblasts adhesiveness to implanted biomaterials, one is to control the surface roughness of the biomaterial and the other is to coat the biomaterial with attachment proteins. Between them, coat the biomaterials with attachment proteins is denounced. Fibronectin and type Ⅰ collagen are the only attachment proteins studied to titanium biomaterials for evaluation of their effectiveness, and the results were not united. And it is difficult to evaluate the exact effect of the attachment proteins due to experimental difficulties and methods of application. So the comparative analytical studies of the effect of attachment proteins under controlled condition are needed. The purpose of this in vitro study was to evaluate the adhesiveness and proliferation of human gingival fibroblasts to attachment protein-coated and non-coated endosseous titanium biomaterials. Well-known attachment proteins were used, they were type Ⅰ collagen, type Ⅳ collagen, fibronectin, laminin, and vitronectin. Each attachment proteins applied onto the commercially pure titanium discs. In this study, the protein-coated and non-coated titanium discs were classified as each groups. Human gingival fibroblasts cultured onto each groups. After 30，60, 180 mins incubation time, unattached cells counted with Coulter counter for evaluation of the adhesiveness of human gingival fibroblasts. The configurations of attached human gingival fibroblasts were done by SEM observation. To confirm the focal contact areas, actin cytochemical and vinculin immunofluorescent staning were done after 3, 24 hours of incubation. To evaluate the effect of attachment proteins on the proliferation of human gingival fibroblasts, 3H-thymidine were added after 72 hours incubation. The results were as follows. 1. Adhesiveness of human gingival fibroblasts were best in fibronectin-coated group among groups (p>0.05). Then laminin, vitronectin, and type Ⅳ collagen groups were better, and there were no statistically significant difference between type Ⅰ collagen group and control (p<0.05). 2. Fibronectin and type Ⅳ collagen groups showed well spread human gingival fibroblasts in SEM observation. 3. Fibronectin group showed most dense and regular arrangement of actin filaments among groups. 4. Fibronectin group showed most distinct vinculin patches along the cytoplasmic process among groups. 5. In the proliferation of human gingival fibroblasts, there were no stasistically significant difference among groups (p<0.05). On the bases of these findings, it is strongly suggest that fibronectin coating at the neck portion of titanium biomaterial promotes adhesiveness of human gingival fibroblasts and suggest the possibility of healthy implant maintenance. Clinical application of the attachment proteins are. further studied and comparative analysis of attachment proteins applied on implant biomaterials against epithelial cells are needed.