The Effect of Propofol on Hypoxic damaged-HaCaT Cells
박창훈, 곽진원, 박봉수, 김영호, 김용덕, 윤지욱, 윤지영, 김철홍,
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박창훈 ( Park Chang-Hoon ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
곽진원 ( Kwak Jin-Won ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
박봉수 ( Park Bong-Soo ) - Pusan National University School of Dentistry Department of Oral Anatomy
김영호 ( Kim Yong-Ho ) - Pusan National University School of Dentistry Department of Oral Anatomy
김용덕 ( Kim Yong-Deok ) - Pusan National University School of Dentistry Department of Oral and Maxillofacial Surgery
윤지욱 ( Yoon Ji-Uk ) - Pusan National University School of Medicine Department of Anesthesiology and Pain Medicine
윤지영 ( Yoon Ji-Young ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
김철홍 ( Kim Cheul-Hong ) - Pusan National University School of Medicine Department of Anesthesiology and Pain Medicine
KMID : 0980220140140010041
Abstract
Background: Autophagy is a self-eating process that is important for balancing sources of energy at critical times in development and in response stress. Autophagy also plays a protective role in removing clearing damaged intracellular organelles and aggregated proteins as well as eliminating intracellular pathogens. The purpose of the present study was to examine the protective effect of propofol against hypoxic damage using keratinocytes.
Methods: Human keratinocytes (HaCaT cells) were obtained from the American Type Culture Collection. Propofol which were made by dissolving them in DMSO were kept frozen at -4℃ until use. The stock was diluted to their concentration with DMEM when needed. Prior to propofol treatment cells were grown to about 80% confluence and then exposed to propofol at different concentrations (0, 25, 50, 75, 100 μM) for 2 h pretreatment. Cell viability was measured using a quantitative colorimetric assay with thiazolyl blue tetrazolium bromide (MTT assay), and fluorescence microscopy and western blot analysis were used for evaluation of autophagy processes.
Results: The viability of propofol-treated HaCaT cells was increased in a dose-dependent manner. Propofol did not show any significant toxic effect on the HaCaT cells. The autophagy inhibitor, 3-methyladenine, reduced cell viability of hypoxia-injured HaCat cells. Fluorescence microscopy and western blot analysis showed propofol induce autophagy pathway signals.
Conclusions: Propofol enhanced viability of hypoxia-injured HaCaT cells and we suggest propofol has cellular protective effects by autophagy signal pathway activation.
키워드
Autophagy; Hacat cell; Hypoxic damage; Propofol
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