Effect of Propofol Preconditioning on Hypoxic-Cultured Human Osteoblast
윤지욱, 신상욱, 박봉수, 김영호, Woo Mi-Na, 윤지영, 김철홍,
소속 상세정보
윤지욱 ( Yoon Ji-Uk ) - Pusan National University School of Medicine Department of Anesthesiology and Pain Medicine
신상욱 ( Shin Sang-Wook ) - Pusan National University School of Medicine Department of Anesthesia and Pain Medicine
박봉수 ( Park Bong-Soo ) - Pusan National University School of Dentistry Department of Oral Anatomy
김영호 ( Kim Yong-Ho ) - Pusan National University School of Dentistry Department of Oral Anatomy
( Woo Mi-Na ) - Pusan National University Dental Hospital Department of Dental Anesthesia and Pain Medicine
윤지영 ( Yoon Ji-Young ) - Pusan National University School of Dentistry Department of Dental Anesthesia and Pain Medicine
김철홍 ( Kim Cheul-Hong ) - Pusan National University School of Medicine Department of Anesthesiology and Pain Medicine
KMID : 0980220140140020107
Abstract
Background: Angiogenesis has been recognized an essential precondition for osteogenesis. Because reduction and disruption of the blood supply to tissue cause tissue hypoxia, pathological bone loss affected by hypoxia often can occur in various clinical conditions. The effects of propofol on the process of osteogenesis have received little direct attention. Therefore, we investigated the effect of propofol on the growth and function of osteoblasts under hypoxic condition.
Methods: After propofol (3, 30, 300 μM) preconditioning for 2 hours, hFOB 1.19 human osteoblast cells were cultured under 1 % oxygen tension for 48 hours. Using real time PCR and western blot analysis, we analyzed the expression of, BMP-2, TGF-β1, type I collagen, osteocalcin, HIF-1s and Akt. Cell viability was also determined by MTT assay.
Results: Propofol preconditioning on hypoxic-cultured osteoblast promoted the expressions of BMP-2, TGF-β1, type I collagen and osteocalcin and induced hypoxia-mediated HIF-1 activation and the expression of Akt protein. Propofol with 300 μM significant decreased cell viability compared to control.
Conclusions: Clinically relevant concentrations of propofol are not cytotoxic to hypoxic osteoblasts in vitro. Propofol preconditioning on hypoxic-cultured osteoblast stimulates proliferation and differentiation of osteoblast through induced expression of BMP-2, TGF-β1, type I collagen and osteocalcin. Propofol might promote angiogenesis and bone regeneration under hypoxic condition.
키워드
ofol; hypoxi; osteoblast
원문 및 링크아웃 정보
등재저널 정보