Èò ÁãÀÇ ÃøµÎÇϾǰüÀý ÅëÁõ¸ðµ¨¿¡¼ Triptolide¿Í N-nitro-L-arginine Methyl EsterÀÇ ÅëÁõÁ¶ÀýÈ¿°ú
Analgesic Effects of Triptolide and N-nitro-L-arginine Methyl Ester in Rat¡¯s Temporomandibular Joint Pain Model
±èÀ±°æ, À̹ΰæ,
¼Ò¼Ó »ó¼¼Á¤º¸
±èÀ±°æ ( Kim Yun-Kyung ) - µ¿ÀÇ´ëÇб³ ÀϹݴëÇпø º¸°ÇÀÇ°úÇаú
À̹ΰæ ( Lee Min-Kyung ) - µ¿ÀÇ´ëÇб³ ÀϹݴëÇпø º¸°ÇÀÇ°úÇаú
KMID : 1023420150150060800
Abstract
ÀÌ ¿¬±¸´Â triptolide¿Í L-NAMEÀÇ ÃøµÎÇϾǰüÀý ÅëÁõÁ¶ÀýÈ¿°ú¸¦ È®ÀÎÇϱâ À§ÇÏ¿© Æ÷¸£¸»¸°À¸·Î À¯µµµÈ ÃøµÎÇϾǰüÀý ÅëÁõ¸ðµ¨¿¡¼ triptolide¿Í L-NAMEÀÇ ¼Ò³ú¿¬¼öÁ¶ ³»°¢°¢ÀÇ ¾â¹°ÀÇ ´Üµ¶ Åõ¿©¿¡ µû¸¥ ÅëÁõÇàÀ§¹ÝÀÀ°ú µÎ ¾à¹°ÀǺ´¿ë Åõ¿©¿¡ µû¸¥ »óÈ£ÀÛ¿ëÀÌ ÅëÁõÇàÀ§¹ÝÀÀ¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» Æò°¡ÇÏ¿© ´ÙÀ½°ú °°Àº °á°ú¸¦ ¾ò¾ú´Ù. ¸ÕÀú, °üÀý° ³»·ÎÁÖÀÔÇÑ 5% Æ÷¸£¸»¸°(30 ¥ìl)Àº À¯ÀÇÇÑ ÅëÁõÇàÀ§¹ÝÀÀÀ» À¯¹ßÇÏ¿´°í, 2Â÷ ÅëÁõÇàÀ§¹ÝÀÀ °üÂû ½Ã Æ÷¸£¸»¸° ÁÖÀÔ Àü 1 ¥ìg/ 10 ¥ìl triptolide Åõ¿©±º(163.33¡¾29.11ȸ)Àº Æ÷¸£¸»¸°±º(308¡¾ 33.04ȸ)°ú ºñ±³ ½Ã ÅëÁõÇàÀ§¹ÝÀÀÀÌ À¯ÀÇÇÏ°Ô °¨¼ÒÇÏ¿´´Ù. 0.1 ¥ìg/10 ¥ìlÀÇ L-NAME Åõ¿©±ºÀÇ 1, 2Â÷ ÅëÁõÇàÀ§¹ÝÀÀÀÇ°á°ú, °¢°¢ 5.80¡¾3.75ȸ, 92.30¡¾16.04ȸ·Î Æ÷¸£¸»¸° ÁÖÀÔ±º 25.4¡¾6.59ȸ, 285.60¡¾29.93ȸ¿Í ºñ±³ ½Ã À¯ÀÇÇÏ°Ô °¨¼ÒµÇ¾ú´Ù. ´ÙÀ½À¸·Î, 1 ¥ìg/10 ¥ìlÀÇ triptolide¿Í 0.01 ¥ìg/10 ¥ìl ÀÇ L-NAME º´¿ë Åõ¿©±º¿¡¼ 1, 2Â÷ ÅëÁõÇàÀ§¹ÝÀÀÀÌ 0.80¡¾ 0.80ȸ, 96.50¡¾26.16ȸ·Î ³ªÅ¸³ª 22.50¡¾19.15ȸ, 163.33¡¾ 29.11ȸ·Î ³ªÅ¸³ 1 ¥ìg/10 ¥ìl trtiptolide±º°ú ºñ±³ ½Ã À¯ÀÇÇÏ°Ô ÅëÁõÇàÀ§¹ÝÀÀÀÌ °æ°¨µÇ¾ú´Ù. ÀÌ·¯ÇÑ ¿¬±¸°á°ú´Â ÃøµÎÇϾǰüÀý ÅëÁõÁ¶ÀýÀÇ ¿¹¹æ ¹× Ä¡·á¿¡ ÀÖ¾î È°¿ë°¡´ÉÇÑ Ãµ¿¬¹°·Î triptolide°¡ Á¦½ÃµÉ ¼ö ÀÖÀ¸¸ç, õ¿¬¹°°ú ÈÇÕ¹°µéÀÇ º´¿ë Åõ¿©¸¦ ÅëÇØ ±× È¿°ú¸¦ Áõ°¡½Ãų ¼ö ÀÖÀ» °ÍÀ¸·Î ±â´ëµÈ´Ù.
The aim of this study was to investigate whether intracisternal administrations of triptolide and N-nitro-L-arginine Methyl Ester (L-NAME) are involved in the regulation of temporomandibular joint (TMJ) pain. The TMJ pain was induced by the injection of 5% formalin (30 ¥ìl) into TMJ capsule of rats. The pain behavioral responses was recorded the number of grooming or scratching on the left TMJ area for 9 successive 5 minutes intervals. Triptolide and L-NAME were administrated intracisternally 10 minutes before formalin injection. The intra-articular injection of formalin produced a biphasic pattern of pain response (first phase: 0¡10 minutes and second phase: 11¡45 minutes). The intracisternal administration of triptolide (1 ¥ìg/10 ¥ìl) and L-NAME (0.1 ¥ìg/10 ¥ìl) suppressed the TMJ pain behavior in each experiment. Co-administration of two drugs was shown the enhanced effect than the analgesic effect by single-administration of triptolide (1 ¥ìg/10 ¥ìl). The triptolide could be a useful analgesic agent for the treatment of TMJ pain, and it is expected to reduce the substantial amount of it via co-administration of synthetic chemical compound and natural products.
Å°¿öµå
Analgesic effect; L-NAME; Pain; Temporomandibular joint; Triptolide
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