Inflammatory Effect of Light-Emitting Diodes Curing Light Irradiation on Raw264.7 Macrophage
정문진, Kil Ki-Sung, 이명화, 이승연, 이혜진, 임도선, 정순정,
소속 상세정보
정문진 ( Jeong Moon-Jin ) - Chosun University School of Dentistry Department of Oral Histology and Developmental Biology
( Kil Ki-Sung ) - Chosun University School of Dentistry Department of Oral Histology and Developmental Biology
이명화 ( Lee Myoung-Hwa ) - Chosun University School of Dentistry Department of Oral Histology and Developmental Biology
이승연 ( Lee Seung-Yeon ) - Chosun University School of Dentistry Department of Oral Histology and Developmental Biology
이혜진 ( Lee Hye-Jin ) - Dong-Pusan College Department of Dental Hygiene
임도선 ( Lim Do-Seon ) - Eulji University Graduate School of Public Health Science Department of Dental Hygiene
정순정 ( Jeong Soon-Jeong ) - Youngsan University College of Health Sciences Department of Dental Hygiene
Abstract
Background: The light-emitting diode (LED) curing light used is presumed to be safe. However, the scientific basis for this is unclear, and the safety of LED curing light is still controversial. The purpose of this study was to investigate the effect of LED curing light irradiation according to the conditions applied for the polymerization of composite resins in dental clinic on the cell viability and inflammatory response in Raw264.7 macrophages and to confirm the stability of LED curing light.
Methods: Cell viability and cell morphology of Raw264.7 macrophages treated with 100 ng/ml of lipopolysaccharide (LPS) or/and LED curing light with a wavelength of 440∼490 nm for 20 seconds were confirmed by methylthiazolydiphenyl-tetrazolium bromide assay and microscopic observation. The production of nitric oxide (NO) and prostaglandin E2 (PGE2) was confirmed by NO assay and PGE2 enzyme-linked immunosorbent assay kit. Expression of interleukin (IL)-1β and tumor necrosis factor (TNF)-α in total RNA and protein was confirmed by reverse transcription polymerase chain reaction and Western blot analysis.
Results: The LED curing light did not affect the viability and morphology of normal Raw264.7 cells but affected the cell viability and induced cytotoxicity in the inflammation-induced Raw264.7 cells by LPS. The irradiation of the LED curing light did not progress to the inflammatory state in the inflammation-induced Raw264.7 macrophage. However, LED curing light irradiation in normal Raw264.7 cells induced an increase in NO and PGE2 production and mRNA and protein expression of IL-1β and TNF-α, indicating that it is possible to induce the inflammatory state.
Conclusion: The irradiation of LED curing light in RAW264.7 macrophage may induce an excessive inflammatory reaction and damage oral tissues. Therefore, it is necessary to limit the long-term irradiation which is inappropriate when applying LED curing light in a dental clinic.
키워드
Inflammatory effect; Light-emitting diode; Light curing light; Macrophage; Raw264.7 cell
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