Journal of Advanced Prosthodontics
Sismanoglu Soner, Demirci Mustafa, Schweikl Helmut, Ozen-Eroglu Gunes, Cetin-Aktas Esin, Kuruca Serap, Tuncer Safa, Tekce Neslihan,
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( Sismanoglu Soner ) - Altinbas University Faculty of Dentistry Department of Restorative Dentistry
( Demirci Mustafa ) - Istanbul University Faculty of Dentistry Department of Restorative Dentistry
( Schweikl Helmut ) - University of Regensburg Medical Centre Department of Operative Dentistry and Periodontology
( Ozen-Eroglu Gunes ) - Istanbul University Aziz Sancar Institute of Experimental Medicine Department of Molecular Medicine
( Cetin-Aktas Esin ) - Istanbul University Aziz Sancar Institute of Experimental Medicine Department of Immunology
( Kuruca Serap ) - Istanbul University Istanbul Faculty of Medicine Department of Physiology
( Tuncer Safa ) - Istanbul University Faculty of Dentistry Department of Restorative Dentistry
( Tekce Neslihan ) - Kocaeli University Faculty of Dentistry Department of Restorative Dentistry
Abstract
PURPOSE: The effects of four different self-adhesive resin cement materials on cell viability and apoptosis after direct and indirect exposure were evaluated using different cell culture techniques.
MATERIALS AND METHODS: Self-adhesive cements were applied to NIH/3T3 mouse fibroblasts by the extract test method, cell culture inserts, and dentin barrier test method. After exposure periods of 24 h and 72 h, the cytotoxicity of these self-adhesive materials was evaluated using the MTT assay (viability) and the Annexin-V-FITC/PI staining (apoptosis).
RESULTS: The lowest cell viability was found in cells exposed to BeautiCem SA for 24 h in the extract test method. Cell viability was reduced to 70.6% compared to negative controls. After the 72 h exposure period, viability rate of cell cultures exposed to BeautiCem SA decreased more than 2- fold (29.5%) while cells exposed to RelyX U200 showed the highest viability rate of 71.4%. In the dentin barrier test method, BeautiCem SA induced the highest number of cells in apoptosis after a 24 h exposure (4.1%). Panavia SA Cement Plus was the material that caused the lowest number of cells in apoptosis (1.5%).
CONCLUSION: The used self-adhesive cements have showed different cytotoxic effects based on the evaluation method. As exposure time increased, the materials showed more cytotoxic and apoptotic effects. BeautiCem SA caused significantly more severe cytotoxic and apoptotic effects than other cements tested. Moreover, cements other than BeautiCem SA have caused necrotic cell death rather than apoptotic cell death.
키워드
Apoptosis; Biocompatibility; Cell culture; Cytotoxicity; Self-adhesive resin cements
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