A comparative evaluation of cytotoxicity of root canal sealers: an in vitro study
Badole Gautam Pyarelal, Warhadpande Manjusha Madhukar, Meshram Ganesh Kothiramji, Bahadure Rakesh Namdeoraoji, Tawani Shubha Gopal, Tawani Gopal, Badole Shital Gautam,
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( Badole Gautam Pyarelal ) - India VSPM’s Dental College and Research Centre Nagpur Department of Conservative Dentistry
( Warhadpande Manjusha Madhukar ) - India Government Dental College & Hospital Department of Conservative Dentistry & Endodontics
( Meshram Ganesh Kothiramji ) - India Peoples Dental College Department of Conservative Dentistry & Endodontics
( Bahadure Rakesh Namdeoraoji ) - India Sharad Pawar Dental College Department of Pedodontics
( Tawani Shubha Gopal ) - India Government Dental College & Hospital Department of Conservative Dentistry & Endodontics
( Tawani Gopal ) - India Government Dental College & Hospital Department of Conservative Dentistry & Endodontics
( Badole Shital Gautam ) - India VSPM’s Dental College and Research Centre Nagpur Department of Conservative Dentistry
KMID : 1034420130380040204
Abstract
Objectives: The objective of this in vitro study was to evaluate and compare the cytotoxicity of four different root canal sealers i.e. Apexit Plus (Ivoclar Vivadent), Endomethasone N (Septodont), AH-26 (Dentsply) and Pulpdent Root Canal Sealer (Pulpdent), on a mouse fibroblast cell line (L929).
Materials and Methods: Thirty two discs for each sealer (5 mm in diameter and 2 mm in height) were fabricated in Teflon mould. The sealer extraction was made in cell culture medium (Dulbecco's Modified Eagle's Medium, DMEM) using the ratio 1.25 cm2/mL between the surface of the sealer samples and the volume of medium in a shaker incubator. Extraction of each sealer was obtained at 24 hr, 7th day, 14th day, and one month of interval. These extracts were incubated with L929 cell line and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay was done. Two-way ANOVA for interaction effects between sealer and time and Post-hoc multiple comparison using Tukey's test across all the 16 different groups were used for statistical analysis.
Results: Apexit Plus root canal sealer was significantly less toxic than other sealers (p < 0.05) and showed higher cellular growth than control. Endomethasone N showed mild cytotoxicity. AH-26 showed severe toxicity which became mild after one month while Pulpdent Root Canal Sealer showed severe to moderate toxicity.
Conclusions: Apexit Plus was relatively biocompatible sealer as compared to other three sealers which were cytotoxic at their initial stages, however, they became biocompatible with time.
키워드
Cell culture; Cytotoxicity; MTT Assay; Root canal sealers
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