Chelating and antibacterial properties of chitosan nanoparticles on dentin
Aldo del Carpio-Perochena,, Bramante Clovis Monteiro, Duarte Marco Antonio Hungaro, de Moura de Moura, Aouada Fauze Ahmad, Aouada Fauze Ahmad, Kishen Anil,
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( Aldo del Carpio-Perochena, ) - University of Sao Paulo Bauru Dental School Department of Dentistry, Endodontics and Dental Materials
( Bramante Clovis Monteiro ) - University of Sao Paulo Bauru Dental School Department of Dentistry, Endodontics and Dental Materials
( Duarte Marco Antonio Hungaro ) - University of Sao Paulo Bauru Dental School Department of Dentistry, Endodontics and Dental Materials
( de Moura de Moura ) - Sao Paulo State University Department of Physics and Chemistry
( Aouada Fauze Ahmad ) - Sao Paulo State University Department of Physics and Chemistry
( Aouada Fauze Ahmad ) - Sao Paulo State University Department of Physics and Chemistry
( Kishen Anil ) - University of Toronto Faculty of Dentistry Discipline of Endodontics
KMID : 1034420150400030195
Abstract
Objectives: The use of chitosan nanoparticles (CNPs) in endodontics is of interest due to their antibiofilm properties. This study was to investigate the ability of bioactive CNPs to remove the smear layer and inhibit bacterial recolonization on dentin.
Materials and Methods: One hundred bovine dentin sections were divided into five groups (n = 20 per group) according to the treatment. The irrigating solutions used were 2.5% sodium hypochlorite (NaOCl) for 20 min, 17% ethylenediaminetetraacetic acid (EDTA) for 3 min and 1.29 mg/mL CNPs for 3 min. The samples were irrigated with either distilled water (control), NaOCl, NaOCl-EDTA, NaOCl-EDTA-CNPs or NaOCl-CNPs. After the treatment, half of the samples (n = 50) were used to assess the chelating effect of the solutions using portable scanning electronic microscopy, while the other half (n = 50) were infected intra-orally to examine the post-treatment bacterial biofilm forming capacity. The biovolume and cellular viability of the biofilms were analysed under confocal laser scanning microscopy. The Kappa test was performed for examiner calibration, and the non-parametric Kruskal-Wallis and Dunn tests (p < 0.05) were used for comparisons among the groups.
Results: The smear layer was significantly reduced in all of the groups except the control and NaOCl groups (p < 0.05). The CNPs-treated samples were able to resist biofilm formation significantly better than other treatment groups (p < 0.05).
Conclusions: CNPs could be used as a final irrigant during root canal treatment with the dual benefit of removing the smear layer and inhibiting bacterial recolonization on root dentin.
키워드
Biofilm; Chitosan; Dentin; Nanoparticles; Recolonization
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