Effect of acidic solutions on the microhardness of dentin and set OrthoMTA and their cytotoxicity on murine macrophage
오소람, Perinpanayagam Hiran, 이윤, Kum Jae-Won, 유연지, 임상민, 장석우, 손원준, 이우철, 백승호, 금기연,
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오소람 ( Oh So-Ram ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
( Perinpanayagam Hiran ) - University of Western Ontario Schulich School of Medicine and Dentistry
이윤 ( Lee Yoon ) - Yonsei University Wonju College of Medicine Department of Conservative Dentistry
( Kum Jae-Won ) - Bendilde St. Margarets School
유연지 ( Yoo Yeon-Jee ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
임상민 ( Lim Sang-Min ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
장석우 ( Chang Seok-Woo ) - Kyung hee University School of Dentistry Department of Conservative Dentistry
손원준 ( Shon Won-Jun ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
이우철 ( Lee Woo-Cheol ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
백승호 ( Baek Seung-Ho ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
금기연 ( Kum Kee-Yeon ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
KMID : 1034420160410010012
Abstract
Objectives: To evaluate the effects of three acids on the microhardness of set mineral trioxide aggregate (MTA) and root dentin, and cytotoxicity on murine macrophage.
Materials and Methods: OrthoMTA (BioMTA) was mixed and packed into the human root dentin blocks of 1.5 mm diameter and 5 mm height. Four groups, each of ten roots, were exposed to 10% citric acid (CA), 5% glycolic acid (GA), 17% ethylenediaminetetraacetic acid (EDTA), and saline for five minutes after setting of the OrthoMTA. Vickers surface microhardness of set MTA and dentin was measured before and after exposure to solutions, and compared between groups using one-way ANOVA with Tukey test. The microhardness value of each group was analyzed using student t test. Acid-treated OrthoMTA and dentin was examined by scanning electron microscope (SEM). Cell viability of tested solutions was assessed using WST-8 assay and murine macrophage.
Results: Three test solutions reduced microhardness of dentin. 17% EDTA demonstrated severe dentinal erosion, significantly reduced the dentinal microhardness compared to 10% CA (p = 0.034) or 5% GA (p = 0.006). 10% CA or 5% GA significantly reduced the surface microhardness of set MTA compared to 17% EDTA and saline (p < 0.001). Acid-treated OrthoMTA demonstrated microporous structure with destruction of globular crystal. EDTA exhibited significantly more cellular toxicity than the other acidic solutions at diluted concentrations (0.2, 0.5, 1.0%).
Conclusions: Tested acidic solutions reduced microhardness of root dentin. Five minute’s application of 10% CA and 5% GA significantly reduced the microhardness of set OrthoMTA with lower cellular cytotoxicity compared to 17% EDTA.
키워드
Citric acid; EDTA; Glycolic acid; OrthoMTA; SEM; Vickers surface microhardness
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