Antifungal effects of synthetic human β-defensin 3-C15 peptide
임상민, 안기범, Kim Christine, Kum Jong-Won, Perinpanayagam Hiran, Gu Yu, 유연지, 장석우, 한승현, 손원준, 이우철, 백승호, Zhu Qiang, 금기연,
소속 상세정보
임상민 ( Lim Sang-Min ) - Seoul National University School of Dentistry Department of Conservative Dentistry
안기범 ( Ahn Ki-Bum ) - Seoul National University School of Dentistry Department of Microbiology and Immunology
( Kim Christine ) - McGill University Department of Anatomy and Cell Biology
( Kum Jong-Won ) - Siena College Department of Biology
( Perinpanayagam Hiran ) - University of Western Ontario Schulich School of Medicine and Dentistry
( Gu Yu ) - Seoul National University School of Dentistry Department of Conservative Dentistry
유연지 ( Yoo Yeon-Jee ) - Seoul National University School of Dentistry Department of Conservative Dentistry
장석우 ( Chang Seok-Woo ) - Kyung hee University School of Dentistry Department of Conservative Dentistry
한승현 ( Han Seung-Hyun ) - Seoul National University School of Dentistry Department of Oral Microbiology and Immunology
손원준 ( Shon Won-Jun ) - Seoul National University School of Dentistry Department of Conservative Dentistry
이우철 ( Lee Woo-Cheol ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
백승호 ( Baek Seung-Ho ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
( Zhu Qiang ) - University of Connecticut Health Center Department of Oral Health and Diagnostic Sciences Division of Endodontology
금기연 ( Kum Kee-Yeon ) - Seoul National University School of Dentistry Seoul National University Dental Hospital Department of Conservative Dentistry
KMID : 1034420160410020091
Abstract
Objectives: The purpose of this ex vivo study was to compare the antifungal activity of a synthetic peptide consisting of 15 amino acids at the C-terminus of human β-defensin 3 (HBD3-C15) with calcium hydroxide (CH) and Nystatin (Nys) against Candida albicans (C. albicans) biofilm.
Materials and Methods: C. albicans were grown on cover glass bottom dishes or human dentin disks for 48 hr, and then treated with HBD3-C15 (0, 12.5, 25, 50, 100, 150, 200, and 300 μg/mL), CH (100 μg/mL), and Nys (20 μg/mL) for 7 days at 37℃. On cover glass, live and dead cells in the biomass were measured by the FilmTracer Biofilm viability assay, and observed by confocal laser scanning microscopy (CLSM). On dentin, normal, diminished and ruptured cells were observed by field-emission scanning electron microscopy (FE-SEM). The results were subjected to a two-tailed t-test, a one way analysis variance and a post hoc test at a significance level of p = 0.05.
Results: C. albicans survival on dentin was inhibited by HBD3-C15 in a dose-dependent manner. There were fewer aggregations of C. albicans in the groups of Nys and HBD3-C15 (≥ 100 μg/mL). CLSM showed C. albicans survival was reduced by HBD3-C15 in a dose dependent manner. Nys and HBD3-C15 (≥ 100 μg/mL) showed significant fungicidal activity compared to CH group (p < 0.05).
Conclusions: Synthetic HBD3-C15 peptide (≥ 100 μg/mL) and Nys exhibited significantly higher antifungal activity than CH against C. albicans by inhibiting cell survival and biofilm.
키워드
Antifungal activity; C. albicans; Confocal laser scanning microscopy; Dentin disk; Human β-defensin-3 peptide; LIVE/DEAD Biofilm viability assay
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