Biocompatibility of two experimental scaffolds for regenerative endodontics
Leong Dephne Jack Xin, Setzer Frank C., Trope Martin, Karabucak Bekir,
소속 상세정보
( Leong Dephne Jack Xin ) - National University of Singapore Faculty of Dentistry
( Setzer Frank C. ) - University of Pennsylvania School of Dental Medicine Department of Endodontics
( Trope Martin ) - University of Pennsylvania School of Dental Medicine Department of Endodontics
( Karabucak Bekir ) - University of Pennsylvania School of Dental Medicine Department of Endodontics
KMID : 1034420160410020098
Abstract
Objectives: The biocompatibility of two experimental scaffolds for potential use in revascularization or pulp regeneration was evaluated.
Materials and Methods: One resilient lyophilized collagen scaffold (COLL), releasing metronidazole and clindamycin, was compared to an experimental injectable poly(lactic-co-glycolic) acid scaffold (PLGA), releasing clindamycin. Human dental pulp stem cells (hDPSCs) were seeded at densities of 1.0 × 104, 2.5 × 104, and 5.0 × 104. The cells were investigated by light microscopy (cell morphology), MTT assay (cell proliferation) and a cytokine (IL-8) ELISA test (biocompatibility).
Results: Under microscope, the morphology of cells coincubated for 7 days with the scaffolds appeared healthy with COLL. Cells in contact with PLGA showed signs of degeneration and apoptosis. MTT assay showed that at 5.0 × 104 hDPSCs, COLL demonstrated significantly higher cell proliferation rates than cells in media only (control, p < 0.01) or cells co-incubated with PLGA (p < 0.01). In ELISA test, no significant differences were observed between cells with media only and COLL at 1, 3, and 6 days. Cells incubated with PLGA expressed significantly higher IL-8 than the control at all time points (p < 0.01) and compared to COLL after 1 and 3 days (p < 0.01).
Conclusions: The COLL showed superior biocompatibility and thus may be suitable for endodontic regeneration purposes.
키워드
Clindamycin; Matrix; Metronidazole; Regenerative endodontics; Revascularization; Scaffold
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