Elemental analysis of caries-affected root dentin and artificially demineralized dentin
성영혜, 손호현, Yi Kee-Wook, 장주혜,
소속 상세정보
성영혜 ( Sung Young-Hye ) - Seoul National University Graduate School Department of Dental Science
손호현 ( Son Ho-Hyun ) - Seoul National University School of Dentistry and Dental Research Institute Department of Conservative Dentistry
( Yi Kee-Wook ) - Korea Basic Science Institute Korea Geochronology Team
장주혜 ( Chang Ju-Hea ) - Seoul National University Seoul National University Dental Hospital Special Care Clinic
KMID : 1034420160410040255
Abstract
Objectives: This study aimed to analyze the mineral composition of naturally- and artificially-produced caries-affected root dentin and to determine the elemental incorporation of resin-modified glass ionomer (RMGI) into the demineralized dentin.
Materials and Methods: Box-formed cavities were prepared on buccal and lingual root surfaces of sound human premolars (n = 15). One cavity was exposed to a microbial caries model using a strain of Streptococcus mutans. The other cavity was subjected to a chemical model under pH cycling. Premolars and molars with root surface caries were used as a natural caries model (n = 15). Outer caries lesion was removed using a carbide bur and a hand excavator under a dyeing technique and restored with RMGI (FujiII LC, GC Corp.). The weight percentages of calcium (Ca), phosphate (P), and strontium (Sr) and the widths of demineralized dentin were determined by electron probe microanalysis and compared among the groups using ANOVA and Tukey test (p < 0.05).
Results: There was a pattern of demineralization in all models, as visualized with scanning electron microscopy. Artificial models induced greater losses of Ca and P and larger widths of demineralized dentin than did a natural caries model (p < 0.05). Sr was diffused into the demineralized dentin layer from RMGI.
Conclusions: Both microbial and chemical caries models produced similar patterns of mineral composition on the caries-affected dentin. However, the artificial lesions had a relatively larger extent of demineralization than did the natural lesions. RMGI was incorporated into the superficial layer of the caries-affected dentin.
키워드
Artificial caries; Caries-affected dentin; Resin-modified glass ionomer (RMGI); Root caries
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