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Effects of proanthocyanidin, a crosslinking agent, on physical and biological properties of collagen hydrogel scaffold

Restorative Dentistry & Endodontics 2016년 41권 4호 p.296 ~ 303
최유리나, 김희진, 민경산,
소속 상세정보
최유리나 ( Choi Yoorina ) - Wonkwang University Dental Hospital Department of Conservative Dentistry
김희진 ( Kim Hee-Jin ) - Chonbuk National University School of Dentistry Department of Conservative Dentistry
민경산 ( Min Kyung-San ) - Chonbuk National University School of Dentistry Department of Conservative Dentistry

Abstract


Objectives: The purpose of the present study was to evaluate the effects of proanthocyanidin (PAC), a crosslinking agent, on the physical properties of a collagen hydrogel and the behavior of human periodontal ligament cells (hPDLCs) cultured in the scaffold.

Materials and Methods: Viability of hPDLCs treated with PAC was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The physical properties of PAC treated collagen hydrogel scaffold were evaluated by the measurement of setting time, surface roughness, and differential scanning calorimetry (DSC). The behavior of the hPDLCs in the collagen scaffold was evaluated by cell morphology observation and cell numbers counting.

Results: The setting time of the collagen scaffold was shortened in the presence of PAC (p < 0.05). The surface roughness of the PAC-treated collagen was higher compared to the untreated control group (p < 0.05). The thermogram of the crosslinked collagen exhibited a higher endothermic peak compared to the uncrosslinked one. Cells in the PAC-treated collagen were observed to attach in closer proximity to one another with more cytoplasmic extensions compared to cells in the untreated control group. The number of cells cultured in the PAC-treated collagen scaffolds was significantly increased compared to the untreated control (p < 0.05).

Conclusions: Our results showed that PAC enhanced the physical properties of the collagen scaffold. Furthermore, the proliferation of hPDLCs cultured in the collagen scaffold crosslinked with PAC was facilitated. Conclusively, the application of PAC to the collagen scaffold may be beneficial for engineering-based periodontal ligament regeneration in delayed replantation.

키워드

Collagen; Crosslinking; Periodontal ligament cell; Proanthocyanidin; Proliferation

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