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Analysis of the shelf life of chitosan stored in different types of packaging, using colorimetry and dentin microhardness

Restorative Dentistry & Endodontics 2017년 42권 2호 p.87 ~ 94
Da Cruz Filho Antonio Miranda, De Vito Bordin Angelo Rafael, Souza Flamini Luis Eduardo, Da Costa Guedes Debora Fernandes, Saquy Paulo Cesar, Silva Ricardo Gariba, Pecora Jesus Djalma,
소속 상세정보
 ( Da Cruz Filho Antonio Miranda ) - University of Sao Paulo Ribeirao Preto Dental School Department of Restorative Dentistry
 ( De Vito Bordin Angelo Rafael ) - University of Sao Paulo Ribeirao Preto Dental School Department of Restorative Dentistry
 ( Souza Flamini Luis Eduardo ) - University of Sao Paulo Ribeirao Preto Dental School Department of Restorative Dentistry
 ( Da Costa Guedes Debora Fernandes ) - University of Sao Paulo Ribeirao Preto Dental School Department of Restorative Dentistry
 ( Saquy Paulo Cesar ) - University of Sao Paulo Ribeirao Preto Dental School Department of Restorative Dentistry
 ( Silva Ricardo Gariba ) - University of Sao Paulo Ribeirao Preto Dental School Department of Restorative Dentistry
 ( Pecora Jesus Djalma ) - University of Sao Paulo Ribeirao Preto Dental School Department of Restorative Dentistry

Abstract


Objectives: Chitosan has been widely investigated and used. However, the literature does not refer to the shelf life of this solution. This study evaluated, through the colorimetric titration technique and an analysis of dentin micro-hardness, the shelf life of 0.2% chitosan solution.

Materials and Methods: Thirty human canines were sectioned, and specimens were obtained from the second and third slices, from cemento-enamel junction to the apex. A 0.2% chitosan solution was prepared and distributed in 3 identical glass bottles (v1, v2, and v3) and 3 plastic bottles (p1, p2, and p3). At 0, 7, 15, 30, 45, 60, 90, 120, 150, and 180 days, the specimens were immersed in each solution for 5 minutes (n = 3 each). The chelating effect of the solution was assessed by micro-hardness and colorimetric analysis of the dentin specimens. 17% EDTA and distilled water were used as controls. Data were analyzed statistically by two-way and Tukey-Kramer multiple comparison (α = 0.05).

Results: There was no statistically significant difference among the solutions with respect to the study time (p = 0.113) and micro-hardness/time interaction (p = 0.329). Chitosan solutions and EDTA reduced the micro-hardness in a similar manner and differed significantly from the control group (p < 0.001). Chitosan solutions chelated calcium ions throughout the entire experiment.

Conclusions: Regardless of the storage form, chitosan demonstrates a chelating property for a minimum period of 6 months.

키워드

Chelators; Chitosan; Colorimetry; Dentin microhardness; Shelf life

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