Removal efficacy and cytotoxicity of a calcium hydroxide paste using N-2-methyl-pyrrolidone as a vehicle
임명진, 장현진, 유미경, 이광원, 민경산,
소속 상세정보
임명진 ( Lim Myung-Jin ) - Chonbuk National University School of Dentistry Department of Conservative Dentistry
장현진 ( Jang Hyun-Jin ) - Chonbuk National University College of Natural Sciences Department of Life Science
유미경 ( Yu Mi-Kyung ) - Chonbuk National University School of Dentistry Department of Conservative Dentistry
이광원 ( Lee Kwang-Won ) - Chonbuk National University School of Dentistry Department of Conservative Dentistry
민경산 ( Min Kyung-San ) - Chonbuk National University School of Dentistry Department of Conservative Dentistry
Abstract
Objectives: This study investigated the removal efficacy and cytotoxicity of a newly developed calcium hydroxide paste (cleaniCal, Maruchi) using N-2-methyl-pyrrolidone (NMP) as a vehicle in comparison with ApexCal (Ivoclar Vivadent) and Calcipex II (Nishika), which use different vehicles such as polyethylene glycol and propylene glycol, respectively.
Materials and Methods: Thirty maxillary premolars with oval-shaped canals were divided into 3 groups and the teeth were filled with one of the pastes. After removal of the paste, micro-computed tomographic (μ-CT) imaging was obtained to assess the volume of residual paste in the root canal of each tooth. The teeth were then split longitudinally and the area of the paste-coated surface was evaluated by stereomicroscopy. The cytotoxicity of each product was assessed using an agar overlay assay. The effect of each vehicle on cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed using one-way analysis of variance and Tukey's tests to detect any significance (p < 0.05).
Results: In the μ-CT and stereomicroscopic analysis, cleaniCal exhibited less remnants of medicament than ApexCal and Calcipex. cleaniCal showed a higher cytotoxicity than the other pastes in the agar overlay assay. Furthermore, NMP exhibited lower cell viability compared to the other vehicles.
Conclusions: cleaniCal showed better removal efficacy compared to the other products. However, clinicians should be aware of the higher cytotoxicity of the NMP-based material and consider its possible adverse effects on periradicular tissue when it is overfilled.
키워드
Calcium hydroxide; Cytotoxicity; N-methylpyrrolidone; Removal
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