Comparison of apical extrusion of intracanal bacteria by various glide-path establishing systems: an in vitro study
Dagna Alberto, Abed Rashid El, Hussain Sameeha, Abu-Tahun Ibrahim H, Visai Livia, Bertoglio Federico, Bosco Floriana, Beltrami Riccardo, Poggio Claudio, 김현철,
소속 상세정보
( Dagna Alberto ) - University of Pavia School of Dentistry Department of Clinical, Surgical, Diagnostic and Pediatric Sciences
( Abed Rashid El ) - Mohammed Bin Rashid University of Medicine and Health Sciences Hamdan Bin Mohammed College of Dental Medicine Endodontic Department
( Hussain Sameeha ) - Dubai Health Authority Department of Dentistry Endodontic Unit
( Abu-Tahun Ibrahim H ) - University of Jordan School of Dentistry Department of Conservative Dentistry
( Visai Livia ) - University of Pavia Department of Molecular Medicine
( Bertoglio Federico ) - University of Pavia Department of Molecular Medicine
( Bosco Floriana ) - University of Pavia School of Dentistry Department of Clinical, Surgical, Diagnostic and Pediatric Sciences
( Beltrami Riccardo ) - University of Pavia School of Dentistry Department of Clinical, Surgical, Diagnostic and Pediatric Sciences
( Poggio Claudio ) - University of Pavia School of Dentistry Department of Clinical, Surgical, Diagnostic and Pediatric Sciences
김현철 ( Kim Hyeon-Cheol ) - Pusan National University School of Dentistry Department of Conservative Dentistry
Abstract
Objectives: This study compared the amount of apically extruded bacteria during the glide-path preparation by using multi-file and single-file glide-path establishing nickel-titanium (NiTi) rotary systems.
Materials and Methods: Sixty mandibular first molar teeth were used to prepare the test apparatus. They were decoronated, blocked into glass vials, sterilized in ethylene oxide gas, infected with a pure culture of Enterococcus faecalis, randomly assigned to 5 experimental groups, and then prepared using manual stainless-steel files (group KF) and glide-path establishing NiTi rotary files (group PF with PathFiles, group GF with G-Files, group PG with ProGlider, and group OG with One G). At the end of canal preparation, 0.01 mL NaCl solution was taken from the experimental vials. The suspension was plated on brain heart infusion agar and colonies of bacteria were counted, and the results were given as number of colony-forming units (CFU).
Results: The manual instrumentation technique tested in group KF extruded the highest number of bacteria compared to the other 4 groups (p < 0.05). The 4 groups using rotary glide-path establishing instruments extruded similar amounts of bacteria.
Conclusions: All glide-path establishment instrument systems tested caused a measurable apical extrusion of bacteria. The manual glide-path preparation showed the highest number of bacteria extruded compared to the other NiTi glide-path establishing instruments.
키워드
Bacterial extrusion; Debris extrusion; Glide-path; Manual instrumentation; Nickel-titanium rotary file
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