Mineral content analysis of root canal dentin using laser-induced breakdown spectroscopy
Eren Selen Kucukkaya, Uzunoglu Emel, Sezer Banu, Yilmaz Zeliha, Boyaci Ismail Hakki,
소속 상세정보
( Eren Selen Kucukkaya ) - Hacettepe University Faculty of Dentistry Department of Endodontics
( Uzunoglu Emel ) - Hacettepe University Faculty of Dentistry Department of Endodontics
( Sezer Banu ) - Hacettepe University Faculty of Engineering Department of Food Engineering
( Yilmaz Zeliha ) - Hacettepe University Faculty of Dentistry Department of Endodontics
( Boyaci Ismail Hakki ) - Hacettepe University Faculty of Engineering Department of Food Engineering
Abstract
Objectives: This study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comparing the effects of irrigation solutions on the mineral content change of root canal dentin.
Materials and Methods: Forty teeth with a single root canal were decoronated and longitudinally sectioned to expose the canals. The root halves were divided into 4 groups (n = 10) according to the solution applied: group NaOCl, 5.25% sodium hypochlorite (NaOCl) for 1 hour; group EDTA, 17% ethylenediaminetetraacetic acid (EDTA) for 2 minutes; group NaOCl+EDTA, 5.25% NaOCl for 1 hour and 17% EDTA for 2 minutes; a control group. Each root half belonging to the same root was evaluated for mineral content with either LIBS or SEM/EDS methods. The data were analyzed statistically.
Results: In groups NaOCl and NaOCl+EDTA, the calcium (Ca)/phosphorus (P) ratio decreased while the sodium (Na) level increased compared with the other groups (p < 0.05). The magnesium (Mg) level changes were not significant among the groups. A significant positive correlation was found between the results of LIBS and SEM/EDS analyses (r = 0.84, p < 0.001).
Conclusions: Treatment with NaOCl for 1 hour altered the mineral content of dentin, while EDTA application for 2 minutes had no effect on the elemental composition. The LIBS method proved to be reliable while providing data for the elemental composition of root canal dentin.
키워드
Dentin; Endodontics; Microscopy; Spectrum analysis
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