Bacterial leakage and micro-computed tomography evaluation in round-shaped canals obturated with bioceramic cone and sealer using matched single cone technique
Yanpiset Kallaya, Banomyong Danuchit, Chotvorrarak Kanet, Srisatjaluk Ratchapin Laovanitch,
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( Yanpiset Kallaya ) - Mahidol University Faculty of Dentistry Department of Operative Dentistry and Endodontics
( Banomyong Danuchit ) - Mahidol University Faculty of Dentistry Department of Operative Dentistry and Endodontics
( Chotvorrarak Kanet ) - Mahidol University Faculty of Dentistry Department of Operative Dentistry and Endodontics
( Srisatjaluk Ratchapin Laovanitch ) - Mahidol University Faculty of Dentistry Department of Oral Microbiology
Abstract
Objectives: To evaluate sealing ability of root canals obturated with bioceramic-impregnated gutta percha cone (BCC) or gutta percha (GP), with bioceramic sealer (BCS) or AH Plus (AH; Dentsply-Maillefer), in roundly-prepared canals using matched single-cone technique, based on bacterial leakage test, and to analyze obturation quality using micro-computed tomography (CT) analysis.
Materials and Methods: Ninety-two distobuccal roots of maxillary molars were prepared using nickel-titanium files to apical size 40/0.06. The roots were divided into 4 groups (n = 20) that were obturated with a master cone and sealer: GP/AH, BCC/AH, GP/BCS, and BCC/BCS. Bacterial leakage model using Enterococcus faecalis was used to evaluate sealing ability for 60-day period. Obturated samples from each group (n = 4) were analyzed using micro-CT.
Results: All groups showed bacterial leakage at 20%?45% of samples with mean leakage times of 42?52 days. There were no significant differences in bacterial leakage among the groups. Micro-CT showed minimal gaps and voids in all groups at less than 1%.
Conclusions: In roundly-prepared canals, the single cone obturation with BCC/BCS was comparable to GP/AH for bacterial leakage at 60 days.
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Bacterial leakage; Bioceramic; Epoxy resin based root canal sealer; Micro computed tomography; Root canal obturation
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