Improved dentin disinfection by combining different-geometry rotary nickel-titanium files in preparing root canals
Bedier Marwa M., Hashem Ahmed Abdel Rahman, Hassan Yosra M.,
소속 상세정보
( Bedier Marwa M. ) - Cairo University Faculty of Dentistry Department of Endodontics
( Hashem Ahmed Abdel Rahman ) - Ain Shams University Faculty of Dentistry Department of Endodontics
( Hassan Yosra M. ) - Cairo University Department of Clinical Pathology
Abstract
Objectives: This study was to evaluate the antibacterial effect of different instrumentation and irrigation techniques using confocal laser scanning microscopy (CLSM) after root canal inoculation with Enterococcus faecalis (E. faecalis).
Materials and Methods: Mesiobuccal and mesiolingual canals of extracted mandibular molars were apically enlarged up to a size 25 hand K-file, then autoclaved and inoculated with E. faecalis. The samples were randomly divided into 4 main groups according to the system of instrumentation and irrigation: an XP-endo Shaper (XPS) combined with conventional irrigation (XPS/C) or an XP-endo Finisher (XPF) (XPS/XPF), and iRaCe combined with conventional irrigation (iRaCe/C) or combined with an XPF (iRaCe/XPF). A middle-third sample was taken from each group, and then the bacterial reduction was evaluated using CLSM at a depth of 50 μm inside the dentinal tubules. The ratio of red fluorescence (dead cells) to green-and-red fluorescence (live and dead cells) represented the percentage of bacterial reduction. The data were then statistically analyzed using the Kruskal-Wallis test for comparisons across the groups and the Dunn test was used for pairwise comparisons.
Results: The instrumentation and irrigation techniques had a significant effect on bacterial reduction (p < 0.05). The iRaCe/XPF group showed the strongest effect, followed by the XPS/XPF and XPS/C group, while the iRaCe/C group had the weakest effect.
Conclusions: Combining iRaCe with XPF improved its bacterial reduction effect, while combining XPS with XPF did not yield a significant improvement in its ability to reduce bacteria at a depth of 50 μm in the dentinal tubules.
키워드
Confocal laser scanning microscopy; Enterococcus faecalis; Instrumentation; Irrigation
원문 및 링크아웃 정보
등재저널 정보