Effects of the cathepsin K inhibitor with mineral trioxide aggregate cements on osteoclastic activity
김희선, 김수정, 고현정, 송민주, 김미리,
소속 상세정보
김희선 ( Kim Hee-Sun ) - Seoul Metropolitan Governance-Seoul National University Boramae Medical Center Department of Conservative Dentistry
김수정 ( Kim Soo-Jung ) - University of Ulsan Asan Medical Center Department of Conservative Dentistry
고현정 ( Ko Hyun-Jung ) - University of Ulsan Asan Medical Center Department of Conservative Dentistry
송민주 ( Song Min-Ju ) - Dankook University School of Dentistry Department of Conservative Dentistry
김미리 ( Kim Mi-Ri ) - University of Ulsan Asan Medical Center Department of Conservative Dentistry
Abstract
Objectives: Root resorption is an unexpected complication after replantation procedures. Combining anti-osteoclastic medicaments with retrograde root filling materials may avert this resorptive activity. The purpose of this study was to assess effects of a cathepsin K inhibitor with calcium silicate-based cements on osteoclastic activity.
Methods: MC3T3-E1 cells were cultured for biocompatibility analyses. RAW 264.7 cells were cultured in the presence of the receptor activator of nuclear factor-kappa B and lipopolysaccharide, followed by treatment with Biodentine (BIOD) or ProRoot MTA with or without medicaments (Odanacatib [ODN], a cathepsin inhibitor and alendronate, a bisphosphonate). After drug treatment, the cell counting kit-8 assay and Alizarin red staining were performed to evaluate biocompatibility in MC3T3-E1 cells. Reverse-transcription polymerase chain reaction, tartrate-resistant acid phosphatase (TRAP) staining and enzyme-linked immunosorbent assays were performed in RAW 264.7 cells to determine the expression levels of inflammatory cytokines, interleukin (IL)-1β, IL-6, tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2). Data were analyzed by one-way analysis of variance and Tukey's post hoc test (p < 0.05).
Results: Biocompatibility results showed that there were no significant differences among any of the groups. RAW 264.7 cells treated with BIOD and ODN showed the lowest levels of TNF-α and PGE2. Treatments with BIOD + ODN were more potent suppressors of inflammatory cytokine expression (p < 0.05).
Conclusion: The cathepsin K inhibitor with calcium silicate-based cement inhibits osteoclastic activity. This may have clinical application in preventing inflammatory root resorption in replanted teeth.
키워드
Cathepsin K; Osteoclastogenesis; Osteoclast; Osteoblast; Root resorption
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