Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers
Mestieri Leticia Boldrin, Zaccara Ivana Maria, Pinheiro Lucas Siqueira, Kopper Patricia Maria Polli, Grecca Fabiana Soares, Barletta Fernando Branco,
¼Ò¼Ó »ó¼¼Á¤º¸
( Mestieri Leticia Boldrin ) - Federal University of Rio Grande do Sul School of Dentistry Department of Conservative Dentistry
( Zaccara Ivana Maria ) - Federal University of Rio Grande do Sul School of Dentistry Department of Conservative Dentistry
( Pinheiro Lucas Siqueira ) - Federal University of Rio Grande do Sul School of Dentistry Department of Conservative Dentistry
( Kopper Patricia Maria Polli ) - Federal University of Rio Grande do Sul School of Dentistry Department of Conservative Dentistry
( Grecca Fabiana Soares ) - Federal University of Rio Grande do Sul School of Dentistry Department of Conservative Dentistry
( Barletta Fernando Branco ) - Lutheran University of Brazil - ULBRA School of Dentistry
Abstract
Objectives: This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus.
Materials and Methods: BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05).
Results: The MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control (p < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control (p > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound (p < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex (p < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing (p > 0.05).
Conclusions: All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.
Å°¿öµå
Root canal; Cell migration; Fibroblast; Endodontics
¿ø¹® ¹× ¸µÅ©¾Æ¿ô Á¤º¸
µîÀçÀú³Î Á¤º¸