A new phantom to evaluate the tissue dissolution ability of endodontic irrigants and activating devices
Khoshroo Kimia, Shah Brinda, Johnson Alexander, Baeten John, Barry Katherine, Tahriri Mohammadreza, Ibrahim Mohamed S., Tayebi Lobat,
소속 상세정보
( Khoshroo Kimia ) - Marquette University School of Dentistry
( Shah Brinda ) - Marquette University School of Dentistry
( Johnson Alexander ) - Inter Med-Vista Dental
( Baeten John ) - Inter Med-Vista Dental
( Barry Katherine ) - Inter Med-Vista Dental
( Tahriri Mohammadreza ) - Marquette University School of Dentistry
( Ibrahim Mohamed S. ) - Marquette University School of Dentistry
( Tayebi Lobat ) - Marquette University School of Dentistry
Abstract
Objective: The aim of this study was to introduce a gelatin/bovine serum albumin (BSA) tissue standard, which provides dissolution properties identical to those of biological tissues. Further, the study evaluated whether the utilization of endodontic activating devices led to enhanced phantom dissolution rates.
Materials and Methods: Bovine pulp tissue was obtained to determine a benchmark of tissue dissolution. The surface area and mass of samples were held constant while the ratio of gelatin and BSA were varied, ranging from 7.5% to 10% gelatin and 5% BSA. Each sample was placed in an individual test tube that was filled with an appropriate sodium hypochlorite solution for 1, 3, and 5 minutes, and then removed from the solution, blotted dry, and weighed again. The remaining tissue was calculated as the percent of initial tissue to determine the tissue dissolution rate. A radiopaque agent (sodium diatrizoate) and a fluorescent dye (methylene blue) were added to the phantom to allow easy quantification of phantom dissolution in a canal block model when activated using ultrasonic (EndoUltra) or sonic (EndoActivator) energy.
Results: The 9% gelatin + 5% BSA phantom showed statistically equivalent dissolution to bovine pulp tissue at all time intervals. Furthermore, the EndoUltra yielded significantly more phantom dissolution in the canal block than the EndoActivator or syringe irrigation.
Conclusions: Our phantom is comparable to biological tissue in terms of tissue dissolution and could be utilized for in vitro tests due to its injectability and detectability.
키워드
Tissue dissolution; Phantom; Endodontic irrigants; Gelatin; Bovine serum albumin
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