Incorporation of amoxicillin-loaded microspheres in mineral trioxide aggregate cement: an in vitro study
Bohns Fabio Rocha, Leitune Vicente Castelo Branco, Garcia Isadora Martini, Genari Bruna, Dornelles Nelio Bairros Junior, Guterres Silvia Staniscuaski, Ogliari Fabricio Aulo, de Melo Mary Anne Sampaio, Collares Fabricio Mezzomo,
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( Bohns Fabio Rocha ) - Federal University of Rio Grande do Sul School of Dentistry Department of Dental Materials
( Leitune Vicente Castelo Branco ) - Federal University of Rio Grande do Sul School of Dentistry Department of Dental Materials
( Garcia Isadora Martini ) - Federal University of Rio Grande do Sul School of Dentistry Department of Dental Materials
( Genari Bruna ) - Federal University of Rio Grande do Sul School of Dentistry Department of Dental Materials
( Dornelles Nelio Bairros Junior ) - Federal University of Rio Grande do Sul School of Dentistry Department of Dental Materials
( Guterres Silvia Staniscuaski ) - Federal University of Rio Grande do Sul School of Pharmaceutical Sciences
( Ogliari Fabricio Aulo ) - Yller Biomaterials
( de Melo Mary Anne Sampaio ) - University of Maryland School of Dentistry Program in Biomedical Sciences
( Collares Fabricio Mezzomo ) - Federal University of Rio Grande do Sul School of Dentistry Department of Dental Materials
Abstract
Objectives: In this study, we investigated the potential of amoxicillin-loaded polymeric microspheres to be delivered to tooth root infection sites via a bioactive reparative cement.
Materials and Methods: Amoxicillin-loaded microspheres were synthesized by a spray-dray method and incorporated at 2.5% and 5% into a mineral trioxide aggregate cement clinically used to induce a mineralized barrier at the root tip of young permanent teeth with incomplete root development and necrotic pulp. The formulations were modified in liquid:powder ratios and in composition by the microspheres. The optimized formulations were evaluated in vitro for physical and mechanical eligibility. The morphology of microspheres was observed under scanning electron microscopy.
Results: The optimized cement formulation containing microspheres at 5% exhibited a delayed-release response and maintained its fundamental functional properties. When mixed with amoxicillin-loaded microspheres, the setting times of both test materials significantly increased. The diametral tensile strength of cement containing microspheres at 5% was similar to control. However, phytic acid had no effect on this outcome (p > 0.05). When mixed with modified liquid:powder ratio, the setting time was significantly longer than that original liquid:powder ratio (p < 0.05).
Conclusions: Lack of optimal concentrations of antibiotics at anatomical sites of the dental tissues is a hallmark of recurrent endodontic infections. Therefore, targeting the controlled release of broad-spectrum antibiotics may improve the therapeutic outcomes of current treatments. Overall, these results indicate that the carry of amoxicillin by microspheres could provide an alternative strategy for the local delivery of antibiotics for the management of tooth infections.
키워드
Aggregate trioxide mineral; Microspheres; Amoxicillin; Dental materials; Endodontics
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