Effects of zinc oxide and calcium?doped zinc oxide nanocrystals on cytotoxicity and reactive oxygen species production in different cell culture models
de Souza Gabriela Leite, Moura Camilla Christian Gomes, Silva Anielle Christine Almeida, Marinho Juliane Zacour, Silva Thaynara Rodrigues, Dantas Noelio Oliveira, Bonvicini Jessica Fernanda Sena, Turrioni Ana Paula,
소속 상세정보
( de Souza Gabriela Leite ) - Federal University of Uberlandia School of Dentistry Department of Endodontics
( Moura Camilla Christian Gomes ) - Federal University of Uberlandia School of Dentistry Department of Endodontics
( Silva Anielle Christine Almeida ) - Federal University of Alagoas Physics Institute
( Marinho Juliane Zacour ) - Federal University of Uberlandia Institute of Chemistry
( Silva Thaynara Rodrigues ) - Federal University of Uberlandia School of Dentistry Department of Endodontics
( Dantas Noelio Oliveira ) - Federal University of Alagoas Physics Institute
( Bonvicini Jessica Fernanda Sena ) - Federal University of Uberlandia School of Dentistry Department of Pediatric Dentistry
( Turrioni Ana Paula ) - Federal University of Uberlandia School of Dentistry Department of Pediatric Dentistry
Abstract
Objectives: This study aimed to synthesize nanocrystals (NCs) of zinc oxide (ZnO) and calcium ion (Ca2+)-doped ZnO with different percentages of calcium oxide (CaO), to evaluate cytotoxicity and to assess the effects of the most promising NCs on cytotoxicity depending on lipopolysaccharide (LPS) stimulation.
Materials and Methods: Nanomaterials were synthesized (ZnO and ZnO:xCa, x = 0.7; 1.0; 5.0; 9.0) and characterized using X-ray diffractometry, scanning electron microscopy, and methylene blue degradation. SAOS-2 and RAW 264.7 were treated with NCs, and evaluated for viability using the MTT assay. NCs with lower cytotoxicity were maintained in contact with LPS-stimulated (+LPS) and nonstimulated (?LPS) human dental pulp cells (hDPCs). Cell viability, nitric oxide (NO), and reactive oxygen species (ROS) production were evaluated. Cells kept in culture medium or LPS served as negative and positive controls, respectively. One-way analysis of variance and the Dunnett test (α = 0.05) were used for statistical testing.
Results: ZnO:0.7Ca and ZnO:1.0Ca at 10 μg/mL were not cytotoxic to SAOS-2 and RAW 264.7. +LPS and ?LPS hDPCs treated with ZnO, ZnO:0.7Ca, and ZnO:1.0Ca presented similar NO production to negative control (p > 0.05) and lower production compared to positive control (p < 0.05). All NCs showed reduced ROS production compared with the positive control group both in +LPS and ?LPS cells (p < 0.05).
Conclusions: NCs were successfully synthesized. ZnO, ZnO:0.7Ca and ZnO:1.0Ca presented the highest percentages of cell viability, decreased ROS and NO production in +LPS cells, and maintenance of NO production at basal levels.
키워드
Cell survival; Nanoparticles; Zinc oxide
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