4-Hexylresorcinol induced angiogenesis potential in human endothelial cells
김민근, 김성곤, 이석근,
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김민근 ( Kim Min-Keun ) - Gangneung-Wonju National University College of Dentistry Department of Oral and Maxillofacial Surgery
김성곤 ( Kim Seong-Gon ) - Gangneung-Wonju National University College of Dentistry Department of Oral and Maxillofacial Surgery
이석근 ( Lee Suk-Keun ) - Gangneung-Wonju National University College of Dentistry Department of Oral Pathology
Abstract
Background: 4-Hexylresorcinol (4HR) is able to increase angiogenesis. However, its molecular mechanism in the human endothelial cells has not been clarified.
Methods: As endothelial cells are important in angiogenesis, we treated the human umbilical vein endothelial cells (HUVECs) with 4HR and investigated protein expressional changes by immunoprecipitation high-performance liquid chromatography (IP-HPLC) using 96 antisera.
Results: Here, we found that 4HR upregulated transforming growth factor-β (TGF-β)/SMAD/vascular endothelial growth factor (VEGF) signaling, RAF-B/ERK and p38 signaling, and M2 macrophage polarization pathways. 4HR also increased expression of caspases and subsequent cellular apoptosis. Mechanistically, 4HR increased TGF-β1 production and subsequent activation of SMADs/VEGFs, RAF-B/ERK and p38 signaling, and M2 macrophage polarization.
Conclusion: Collectively, 4HR activates TGF-β/SMAD/VEGF signaling in endothelial cells and induced vascular regeneration and remodeling for wound healing.
키워드
4HR; HUVEC; IP-HPLC; TGF-β1; Angiogenesis
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