A Study Comparing the Characteristics of Zinc Oxide Eugenol-Based and Mineral Trioxide Aggregate-Based Root Canal Sealers
이석은, 조자원, 유현준, 이명구, 전열매, 김다희, 박혜원,
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이석은 ( Lee Seok-Eun ) - Dankook University College of Dentistry Department of Preventive Dentistry
조자원 ( Cho Ja-Won ) - Dankook University College of Dentistry Department of Preventive Dentistry
유현준 ( Yoo Hyun-Jun ) - Dankook University College of Dentistry Department of Preventive Dentistry
이명구 ( Lee Myung-Gu ) - Konkuk University College of Biomedical & Health Science Department of Biomedical Chemistry
전열매 ( Jeon Yeol-Mae ) - Dankook University College of Dentistry Department of Preventive Dentistry
김다희 ( Kim Da-Hui ) - Dankook University College of Health Science Department of Dental Hygiene
박혜원 ( Park Hye-Won ) - Dankook University College of Dentistry Department of Preventive Dentistry
Abstract
Objective: This study aimed to investigate the antimicrobial activity and cytotoxicity of zinc oxide eugenol (ZOE)-based and mineral trioxide aggregate (MTA)-based root canal sealers.
Methods: The root canal sealers used in this study were Tubli-Seal (a ZOE-based sealer) and ProRoot (an MTA-based sealer). The antimicrobial activity of ProRoot and Tubli-seal was assessed using a susceptibility assay using Enterococcus faecalis, Porphyromonas endodontalis, and Porphyromonas gingivalis. The cytotoxicity of the root canal sealers was investigated using MTT assay, real-time RT-PCR, and ELISA after the pulp cells were treated with sealers. Additionally, the components of the root canal sealers were analyzed using X-ray fluorescence.
Results: The MTA-based sealer showed stronger antimicrobial activity against E. faecalis than the ZOE-based sealer, and the antimicrobial activities of both sealers against P. endodontalis and P. gingivalis were not significantly different. The ZOE-based sealer showed strong cytotoxicity towards pulp cells and induced the expression and production of inflammatory cytokines. However, the MTA-based sealer did not affect the viability of pulp cells or significantly induce the expression of inflammatory cytokines such as TNF-α, IL-6, and IL-8. In the component analysis, the MTA-based sealer had more kinds of metal oxides than the ZOE-based sealer.
Conclusion: The MTA-based sealers had stronger antimicrobial activity against E. faecalis compared to the ZOE-based sealer, and did not show significant cytotoxicity towards pulp cells. Based on these results, MTA-based sealers may be a suitable material for root canal therapy.
키워드
mineral trioxide; root canal; sealers; zinc oxide eugenol
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