Epigallocatechin-3-gallate 농도가 치주인대 세포의 생활력에 미치는 효과
PDL cell viability according to concentrations of epigallocatechin-3-gallate
유은규, 남옥형, 김미선, 이효설, 김광철, 최성철,
소속 상세정보
유은규 ( Yoo Eun-Kyu ) - Kyung Hee University School of Dentistry Department of Pediatric Dentistry
남옥형 ( Nam Ok-Hyung ) - Kyung Hee University School of Dentistry Department of Pediatric Dentistry
김미선 ( Kim Mi-Sun ) - Kyung Hee University Dental Hospital at Gangdong Department of Pediatric Dentistry
이효설 ( Lee Hyo-Seol ) - Kyung Hee University School of Dentistry Department of Pediatric Dentistry
김광철 ( Kim Kwang-Chul ) - Kyung Hee University Dental Hospital at Gangdong Department of Pediatric Dentistry
최성철 ( Choi Sung-Chul ) - Kyung Hee University School of Dentistry Department of Pediatric Dentistry
KMID : 1812220180010010005
Abstract
Purpose: The aim of this study was to evaluate the effect of epigallocatechin?3?gallate (EGCG) as a storage media on the viability of human periodontal ligament (PDL) cell.
Materials and Methods: Human PDL cells were collected and incubated from the healthy premolars that were extracted for orthodontic treatment. The cultures were exposed at tap water, HBSS, and EGCG (1, 5, 10, 20, 50, and 100 μM) for 24 hrs. The viability of PDL cells was determined using MTS assay and the survival rate was counted by Nucleocounter system.
Results: PDL cells in less than 5 μM EGCG showed a similar rate of cell viability compared to HBSS (p > 0.05). However, PDL cells in 10, 20, and 50 μM EGCG showed lower rates compared to the HBSS. PDL cells in 100 μM EGCG and tap water demonstrated remarkably decreased rate of viability (p < 0.05). Survival rates of PDL cells in less than 20 μM EGCG was similar with HBSS (p > 0.05). Survival rate of PDL cells in 50 μM EGCG was decreased after 3 hr (p < 0.05). Also, survival rates of PDL cells in 100μM EGCG and tap water were significantly low (p < 0.05).
Conclusion: PDL cells stored in less than 20 μM appear to be effective concentration for preserving the viability of PDL cells.
키워드
Epigallocatechin-3-gallate; periodontal ligament cell; avulsion; storage medium
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